Ukukhetha nokuqhathanisa indlela yokujova amagciwane

Ukukhetha nokuqhathanisa indlela yokujova amagciwane

Ziningi izindlela zokujova amabhaktheriya, njenge-streak method, indlela yokumboza, indlela yokuthela, indlela yokugoma, indlela ye-liquid culture medium inoculation, indlela ye-spiral inoculation, njll. Izindlela nezindlela zokusebenza zihlukile.Izincazelo ezilandelayo zizosiza abahloli.Khetha isenzo.

Indlela ye-streak: Le ndlela isetshenziselwa ikakhulukazi ukuhlanzwa kwezinhlobo ukuze kutholwe ikoloni eyodwa.
Gcoba kancane okokusebenza okuzohlukaniswa neiluphu ye-inoculation, futhi yenze ukubhala okuhambisanayo, ukubhala okumise okunjengefeni noma ezinye izinhlobo zokubhala okuqhubekayo ebusweni bepuleti eliyinyumba.Manje, uma i-streak ifanelekile, ama-microorganisms angahlakazwa ngamunye ngamunye, futhi ngemva kokulima, ikholoni eyodwa ingatholakala ebusweni bepuleti.
Izinzuzo: Izici ze-Colony zingabonwa futhi amagciwane axubile angahlukaniswa.
Ububi: Ayikwazi ukusetshenziselwa ukubala ikholoni.
 
Indlela yokumboza: Le ndlela isetshenziswa kakhulu ukubala inani eliphelele lamakoloni
Qala ngokuncibilikisa okuphakathi bese uwuthela epuletini eliyinyumba lisashisa, bese usebenzisa ipayipi eliyinyumba ukuze udwebe u-0.1 ml wesisombululo sebhaktheriya bese uwujova epuleti le-agar eliqinile.Bese usebenzisa induku yengilazi eyinyumba emise okuka-L ukuze ugcobe uketshezi lwebhaktheriya epuletini ngokulinganayo, wendlale ipuleti eligcotshiwe phezu kwetafula imizuzu engu-20-30, ukuze uketshezi lwebhaktheriya lungene endaweni yesiko, bese uguqula ipuleti, ugcine. ukufukamela isikhathi eside.Ingabalwa ngemva kokuba amagciwane esephumile.
Izinzuzo: zingabalwa futhi izici zekoloni zingabonwa.
Ukungalungi: i-gradient dilution iyadingeka ngaphambi kokujova, ukumuncwa kuncane, okuyinkinga kakhulu, ipuleti alomi kahle, futhi kulula ukusabalalisa.

Indlela yokulahla: Le ndlela isetshenziswa kakhulu ukubala inani eliphelele lamakoloni
Faka i-1ml yoketshezi lwebhaktheriya epuletini, uthele isiko lebhaktheriya elincibilikisiwe nelipholile libe ngu-45~50°C, uphendukise kahle ipuleti ukuze uhlanganise uketshezi olunamagciwane kanye nendawo ngokulinganayo, guqula ngemva kokupholisa, futhi ulime ezingeni lokushisa elifanele.Ingabalwa ngemva kokuba ikholoni selikhulile.
Izinzuzo: ingabalwa, ilula kakhulu.
Ukungalungi: ukuhlanjululwa kwe-gradient kuyadingeka ngaphambi kokujova, izici zekholoni azikwazi ukubonwa, futhi akufanelekile kubhaktheriya eqinile ye-aerobic kanye namagciwane azwela ukushisa.
 
Indlela yokujova emthambekeni: Le ndlela isetshenziswa kakhulu ukulondoloza izinhlobo zamabhaktheriya, noma ukuhlola izici ezithile ze-biochemical kanye ne-kinetics yamagciwane.
Sebenzisa iiluphu ye-inoculatingnoma inaliti yokujova ukuze ingene epayipini lokujova futhi ikhethe amakoloni asetshenziselwa ukujova.Nweba kushubhu yesiko ethambekele, qala uhudule umugqa wokujova ukusuka phansi kwendawo ethambekele ukuya phezulu, bese ugoqa umugqa ukusuka phansi ukuya phezulu, noma ugoqe ngokuqondile ukusuka phansi ukuya phezulu.Ngemva kokuphothula ukujova, umlomo weshubhu yesiko ukhishwa inzalo ngelangabi, ipulaki likakotini liyaxhunywa, futhi likhuliswe ku-37°C.
 
Indlela yokujova okuphakathi kwesiko eliwuketshezi: Le ndlela isetshenziswa kakhulu ekuhloleni ukuqunjelwa koketshezi lwebhaktheriya.
Khetha amakholomu noma ama-specimens nge-loop yokufaka inzalo, bese ugaya ngobumnene lapho kuhlangana khona udonga lwangaphakathi lweshubhu lokuhlola kanye nendawo ewuketshezi ukuze wenze amagciwane ahlakazeke ngokulinganayo endaweni ewuketshezi.

Bacterial inoculation method selection and comparison


Isikhathi sokuthumela: Apr-12-2022

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