Ungakukhetha kanjani okusebenzisekayo kwelabhorathri ye-PCR (2)

Hukukhetha okusebenzisekayo kwelabhorethri ye-PCR (2)

Ukubhebhetheka kwalolu bhubhane olusha kwezinye izindawo sekuyinto ekhathaza kakhulu wonke umuntu.Ukutholwa kwe-coronavirus entsha njengamanje kusetshenziswa kakhulu indlela ye-PCR ye-fluorescent quantitative, futhi isikhathi sokuphothulwa kombiko wokuhlolwa kwe-nucleic acid sithatha cishe amahora ama-4 kuye kwayi-5.I-Viral nucleic acid ingasetshenziselwa ukutholwa kuphela ngemuva kokukhipha.Ngaphambi kokwenza ukuhlolwa kwensimbi, kuyadingeka ukuthi udlule emsebenzini wokwakha isistimu ngaphambi kokufakwa kokuhlolwa kwe-PCR emshinini, bese udlulisela emshinini we-PCR ukuze uqale ukuhlolwa kwe-PCR.

Kuyo yonke inqubo, ukwengeza uketshezi ku-PCR kubonakala kuwumsebenzi olula, kodwa esigabeni samanje lapho inani lokutholwa landa kakhulu, empeleni kuyisixhumanisi esidinga abasebenzi.Abasebenzisi bamapayipi ngokuvamile banokuningi okucatshangwayo lapho bekhetha i-pipette yokulinganisa, kodwa lapho bekhetha ithiphu le-pipette yokulinganisa,'ukukunaka ngokwanele.Ithiphu ye-pipette yokulinganisa ifana nesibhamu sokulinganisa isibhamu se-pipette, esinomthelela omkhulu ekunembeni kwepayipi, ngakho-ke ukubaluleka akulahleki.ukulinganisa isibhamu se-pipette.Mncanyana, buza enyuvesi, sifunde ndawonye.

PCR 检测 800

1 Yiluphi ulwazi okudingeka silwazi ukuze sithenge ithiphu?

“Inqobo nje uma ithiphu elingafakwa liyithiphu elingasetshenziswa”-lokhu cishe ukuqonda okuvamile kwabasebenzisi mayelana nokuvumelana nezimo kwethiphu.Lesi sitatimende singashiwo siyiqiniso ngokwengxenye kodwa singelona iqiniso ngokuphelele.

Ithiphu engafakwa ku-pipette yokulinganisa ngempela ingakha uhlelo lwamapayipi ngepayipi lokulinganisa lokulinganisa ukuze kubonakale umsebenzi wepayipi, kodwa ingabe lokhu kuthembekile?Kudingeka uphawu lombuzo lapha.Ukuphendula lo mbuzo kudinga idatha ukuze ukhulume.

(1)Ungase ufise ukwenza ukuhlolwa kokusebenza ngemva kokufanisa ipayipi lokulinganisa nethiphu.Ngemva kokugeza ithiphu, yenza imisebenzi eminingi yokwengeza isampula ephindaphindiwe, kala inani lesampula lesengezo isikhathi ngasinye, bese urekhoda okufundiwe.

(2)Bala ukunemba nokunemba komsebenzi wamapayipi ngemva kokuwuguqula ube yivolumu ngokuya ngokuminyana koketshezi lokuhlola.

(3)Okufanele sikukhethe yithiphu enembayo enhle nenembe kakhudlwana.Uma i-pipette yokulinganisa yokulinganisa kanye ne-pipette yokulinganisa ihambisana nokunemba okungalungile, kusho ukuthi ukuqina kwe-pipette yokulinganisa kanye ne-pipette yokulinganisa ayikwazi ukuqinisekiswa, ukuze imiphumela yomsebenzi ngamunye ayikwazi ukukhiqizwa kabusha.

 

2 Yiluphi ulwazi okudingeka silwazi ukuze sithenge ithiphu?

Izinkomba zokusebenza zethiphu yilezi: i-adsorption ephansi, isici sokuhlunga, ukuqina, amandla okulayisha nokukhipha, ayikho i-DNase ne-RNase, ayikho i-pyrogen;Lezi zinkomba zingatholakala kulolu lwazi olulandelayo.

 

01 Ikhwalithi yezinto ezibonakalayo Njengamanje, amathiphu okumunca emakethe ngokuyisisekelo enziwe ngepulasitiki ye-polypropylene (ipulasitiki engenambala nesobala enokungangeni kwamakhemikhali aphezulu kanye nebanga lokushisa elibanzi).Amathiphu ekhwalithi ephezulu ngokuvamile enziwa nge-polypropylene yemvelo, kuyilapho amathiphu ashibhile angase asebenzise ipulasitiki ye-polypropylene egaywe kabusha.Ithiphu ehlinzekwe ngu-Huida yenziwe ngezinto ezibonakalayo ze-polypropylene ezingenisiwe ezihlangabezana nezindinganiso ze-USP Class-VI.Inokucaca okuphezulu, ukumelana nezinyibilikisi eziphilayo, kanye nezinhlobonhlobo zokumelana nezinga lokushisa: -80~121, akukho deformation ngemva kokushisa okuphezulu nokucindezela okuphezulu, izinto zokusetshenziswa ezisezingeni eliphezulu Letha ukusebenza okuphezulu!

Ngaphezu kwalokho, amathiphu amaningi azokwengeza inani elincane lezithasiselo phakathi nenqubo yokukhiqiza.Ezivamile yilezi:

(1) izinto ezithuthukisa umbala, ezisetshenziselwa amathiphu aluhlaza okwesibhakabhaka nokuphuzi;

(2) izithasiselo.Siza ithiphu ukuba ihlukaniswe nesikhunta ngokushesha ngemva kokubunjwa.

(3) Izithasiselo zokwelapha ingaphezulu lethiphu yesibhamu se-adsorption ephansi.

 

02 Ukungcola kwezinto eziphilayo

Abasebenzisi abaningi ngokwengeziwe bazodinga ukuthi amathiphu awanayo i-RNase, i-DNase-free, i-DNA-free, i-pyrogen-free kanye ne-ATP.Kodwa-ke, amathiphu afakwe inzalo yokushisa okuphezulu awakwazi ukuqinisekisa ngokuphelele ukungabikho kokungcoliswa kwebhayoloji.Ukuqinisekisa ukuthi akukho ukungcoliswa kwezinto eziphilayo, izinyathelo ezifana nokwelashwa kwe-gamma ray ziyadingeka.Ngakho-ke, cishe akunakwenzeka ukuthi abasebenzisi balahle amathiphu ajwayelekile kumathiphu angangcolisiwe ngokwebhayoloji, futhi amathiphu athengiwe asebhokisini angangcolisiwe ngokwebhayoloji kufanele ahlinzeke ngezitifiketi ezihambisanayo ukukhombisa idatha yokuhlola yezinto ezihambisanayo.Amathiphu ahlinzekwe ngu-Doheng wonke akhiqizwa endaweni yokusebenzela engenathuli yekilasi engu-100,000 ukuze kuqinisekiswe ukuthi imikhiqizo ayinayo i-pyrogen, i-DNase-free, kanye ne-RNase.

 

03Isigaba samathiphu kanye nokucaciswa

Ngokungeziwe kumathiphu ajwayelekile asetshenziswa kakhulu, kukhona namathiphu okuhlunga, amathiphu e-adsorption ephansi, amathiphu okusebenza okuzenzakalelayo, namathiphu omlomo obanzi, afanele ukuhlolwa okuhlukile.Isibonelo, isampula yokulinganisa ipayipi efakwe ithiphu lesihlungi ayikwazi ukungena ngaphakathi kwe-pipette yokulinganisa, ngaleyo ndlela ivikele izingxenye ze-pipette yokulinganisa ekungcoleni nasekugxumeni.Okubaluleke nakakhulu, ingaqinisekisa futhi ukuthi ngeke kube khona ukungcoliswa okuphambene phakathi kwamasampuli.

Imininingwane yokupakisha ithiphu ikakhulukazi ezikhwameni nasemabhokisini.Ibhokisi livalwa inzalo ngaphambi kokushiya imboni, futhi umsebenzisi angalisebenzisa ngokuqondile;kunamathiphu angu-500 noma angu-1000 esikhwameni ngasinye, futhi abasebenzisi abaningi bazothenga amathiphu afakwe esikhwameni bese befaka amathiphu ebhokisini lethiphu.Ukupakishwa kwebhokisi kuyabiza kakhulu, kodwa ukupakishwa kwezikhwama nakho kwandisa ingcuphe yokungcola phakathi nenqubo yokulayisha mathupha ibhokisi lethiphu.

 

I-Huida inohlu oluphelele lwamathiphu, kokubili ezikhwameni nasemabhokisini.Ukucaciswa kwamapayipi: 10ul, 100ul, 200ul, 300ul, 1000ul, 1250ul;kukhona namathiphu ajwayelekile, amathiphu okuhlunga, namathiphu okuqhuba afanele izindawo zokusebenza..Njengomphakeli ochwepheshe wemishini yezokwelapha nezinto ezisetshenziswayo, u-Huida akakwazi nje ukunikeza amakhasimende izixazululo eziphelele zamathuluzi nemishini ye-PCR, futhi alwele izinto ezisetshenziswa elabhorethri ye-PCR, singanikeza uhla olugcwele lwemikhiqizo yekhwalithi ephezulu kanye nezinsizakalo ezihlobene.Siyakwamukela ukuthumela umbuzo ukuze uthole imininingwane eyengeziwe.

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Isikhathi sokuthumela: Jun-01-2021

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