Beka ucezu lwephepha lokuhlunga eliyindilinga phansipetri dish, beka ingilazi emise okwe-U phezu kwayo, bese ubeka amabili ahlanzekileingilazi amaslayidiphezu kwenduku yengilazi.
Ngemuva kokuvala inzalo, engeza amamililitha ambalwa esixazululo esivunyeleka uwoyela esingu-20% ephepheni lokuhlunga ngendlela yase-aseptic.Umsebenzi wawo oyinhloko ukugcina iphepha lokuhlunga limanzi phakathi nenqubo yesiko.
Usebenzisa i-Pasteur pipette, engeza i-agar yeast encibilikisiwe noma i-potato dextrose agar ebusweni besilayidi sengilazi ukuze wenze ungqimba oluncane lwe-agar medium.
Sebenzisa iiluphu ye-inoculatingukuze ukhiphe imvubelo ukuze ihlolwe esilayidini engilazi, vala ingxenye yesiko le-culture ngengilazi yekhava oyinyumba, umboze isitsha se-petri bese usibeka ku-25 ° C izinsuku ezingaphezu kwezingu-7.
Sebenzisa isibonakhulu sokugqama kwesigaba ukuze ubone ukukhula kwamagciwane esigabeni sokukhula, futhi uqhathanise ukukhula kwamagciwane achayeke emoyeni nangaphansi kwengilazi yekhava.Ungakwazi futhi ukususa i-ikhava ingilazikusukela ku-slide engilazi, phonsa iconsi le-lactophenol, i-lactophenol-picric acid noma i-lactophenol ukotini eluhlaza okwesibhakabhaka ukuze ulungise amagciwane, bese uvala ingilazi yekhava ngopharafini, i-nail polish noma ama-Special adhesive seals.
1. Sula isilayidi bese uvala ingilazi.
2. Beka iconsi lamanzi phakathi nendawo ye-slide yengilazi.
3. Sebenzisa inaliti yokuhlinza ukuze ucoshe ama-hyphae ambalwa.
4. Faka isicelo kumaconsi amanzi acwebile.
5. Mboza ngengilazi yekhava.
6. Ukudaya kudinga ukwenziwa, futhi kungabonwa ngaphandle kokudaya.
Njengepetri dish,ingilazi amaslayidi, iluphu yokujova, ikhava ingilazi umkhiqizi & umphakeli wemishini yezokwelapha kanye nemishini, i-Huida ayikwazi nje ukunikeza amakhasimende izixazululo eziphelele zemishini yaselabhorethri, kodwa futhi inikeze uhla oluphelele lwemikhiqizo yekhwalithi ephezulu kanye nezinsizakalo ezihambisanayo zamalebhu zelabhorethri.Siyakwamukela ukuthumela umbuzo ukuze uthole imininingwane eyengeziwe.
Isikhathi sokuthumela: Jun-09-2021